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Introduction
2D DIGE image overlays enable easy visual comparison between two protein samples labeled with Cy3 and Cy5 and presented in green and red colors. The more mature B cell type is presented in red color. Triple image overlays enable comparison between the two samples in relative to internal standard sample (Stnd) labeled with Cy2 and presented in blue color.
Explanation for colors in double image overlays:
Yellow: Proteins shared between the two samples.
Green and red: Unique proteins in the sample. In Ramos samples (Gels 5-7) red indicates up-regulation and green down-regulation in response to anti-IgM stimulation.
Triple image overlays:
White: Proteins shared between the three samples (no difference in protein abundance).
Yellow: Proteins shared between the two samples. Protein abundance is high in the two samples in relative to Stnd.
Blue: Protein abundance is negligible in relative to Stnd.
Green and red: Unique proteins in the sample. Protein abundance is high in relative to Stnd and to the other sample.
Turquoise and purple: Protein abundance is higher/lower in relative to the other sample.
Highlighted
Proteins highlighted in red are identified either by PMF or by combining MS and MS/MS spectral data for database searches. Proteins highlighted in blue are identified by comparing their positions on 2D DIGE gel to known proteins in other lymphocyte 2DE databases. Move the mouse pointer over a spot to see the protein name and number referring to Table 1 and Supplementary Tables 2-3. Click on the spot to get UniProt information about the protein.
Original view
Interactive 2D DIGE map. B cell protein samples labeled with Cy3 and Cy5 and separated in nonlinear pH gradient 3-10. Internal standard is labeled with Cy2 and run on the same gel.
© 2012
University of Tampere
IBT Bioinformatics
Finland
Lund University
Department of Experimental Medical Science
Sweden
Interactive 2D DIGE maps
Ramos 120 h and 380 proteome samples labeled with Cy3 and Cy5 dyes, respectively, and separated in nonlinear pH gradient 3-10. Internal standard (Stnd) is pooled reference sample labeled with Cy2 and run on the same gel. Move the mouse pointer over a spot to see the protein name and number referring to Table 1 and Supplementary Tables 2-3. Click on the spot to get UniProt information about the protein.
380 (early pre-B phenotype) compared to Ramos 120 h (immature B cell phenotype) in colors:
Double image overlay enable easy visual comparison between 380 (Cy5 green) and Ramos 120 h (Cy3 red). Green proteins are unique to 380 whereas red proteins are unique to Ramos. Red/green indicates that protein abundance is higher/lower in immature B compared to early pre-B cell. Yellow proteins are shared between 380 and Ramos (no difference in protein abundance).
Triple image overlay enable comparison between 380 (Cy5 green) and Ramos 120 h (Cy3 red) in relative to Stnd (Cy2 blue). Red and green proteins are highly expressed in Ramos and 380, respectively. Blue proteins are negligibly expressed in Ramos and 380 relative to Stnd. White proteins are shared between the three samples (no difference). Yellow proteins are highly expressed in Ramos and 380 relative to Stnd. Turquoise and purple proteins are negligibly expressed in Ramos and 380, respectively.